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Testosterone (17ß-hydroxyandrost-4-en-3-one) is an anabolic steroid synthesized primarily by Leydig cells in the testes of male, the ovary of female, and adrenal glands of both sexes. It is synthesized from cholesterol, androstenediol, Dehydroepiandrosterone (DHEA), progesterone, and pregnenolone acting as some of the intermediate substrates. Testosterone level in male increase 10 to 20-fold during puberty, driving the physiological changes associated with male puberty. It also exerts a powerful, wide-ranging influence over emotional well-being, sexual function, muscle mass and strength, energy, cardiovascular health, bone integrity, and cognitive ability throughout a man’s entire life. In the blood only 1 to 15% of testosterone is in its unbound or biologically active form. The remaining testosterone is bound to serum proteins.